Premium
Three‐dimensional imaging of walleye pollock otoliths: reconstruction from serial sections and fluorescent laser cytometry
Author(s) -
Bailey K. M.,
Brown A. L.,
Nishimura A.,
Reilly M. T.
Publication year - 1995
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1995.tb01933.x
Subject(s) - otolith , biology , fish <actinopterygii> , fluorescence , pollock , cytometry , anatomy , fishery , optics , flow cytometry , microbiology and biotechnology , physics
Two techniques have been developed to examine the three‐dimensional internal structure of otoliths. In the first, otoliths were sectioned serially, images were digitized, and the otolith was reconstructed as a computer model. In the second method growth increments were marked in vivo during their formation by immersing the fish in a fluorescent dye, and then the internal structure of the otolith visualized using laser cytometry. The results are useful for evaluating the potential for bias in otolith measurements and for determining the sectional plane with the least bias.