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Organogenesis of the liver in sea bream
Author(s) -
Guyot E.,
Diaz J. P.,
Connes R.
Publication year - 1995
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1995.tb01912.x
Subject(s) - bone canaliculus , biology , glycogen , hatching , coelom , cytoplasm , secretion , anatomy , hepatocyte , microbiology and biotechnology , endocrinology , biochemistry , ecology , in vitro
Development of the liver in sea bream, Sparus aurata , was studied using light and electron microscopy from hatching to the age of 23 days. Histochemical reactions were used to monitor changes in lipids and glycogen during this period. During the strictly endotrophic prelarval phase from hatching until mouth‐opening, the primordial liver formed by budding on the gut wall and became organized in contact with the vitelline vesicle. The initially undifferentiated cells rapidly polarized and became pyramidal in outline with their apical extremities terminating in cavities corresponding to the future bile canaliculi. Sinusoids formed centripetally. Glycogen was stored in the cytoplasm of differentiating hepatocytes. At the beginning of the larval stage the hepatocytes multiplied, bile canaliculi were completed and the sinusoids acquired their final form. Glycogen reserves decreased strongly as bile secretion started. The end of the endo‐extrophic period was marked by distinct recovery of glycogen storage, synthesis of numerous lipoproteins and discharge into the sinusoids.