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Mitochondrial DNA variation in River Usk brown trout, Salmo trutta
Author(s) -
Bembo D. G.,
Weightman A. J.,
Beverton R. J. H.,
Cresswell R. C.
Publication year - 1994
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1994.tb01247.x
Subject(s) - brown trout , salmo , biology , mitochondrial dna , fish migration , stocking , trout , restriction enzyme , restriction fragment length polymorphism , population , zoology , genotype , genetics , fishery , fish <actinopterygii> , dna , gene , demography , sociology
Mitochondrial DNA (mtDNA) polymorphism in the essentially non‐anadromous River Usk brown trout Salmo trutta population was investigated by restriction analysis. Following mtDNA extraction and purification on caesium chloride density gradients, monomorphic restriction profiles were obtained with Hae III , Hind III , Sau 3AI and Xbal . However, the restriction endonucleases Ava II and HinfIl proved informative. The distribution of four composite genotypes found within the Usk system was heterogeneous, and a fifth genotype appeared exclusively in an outgroup sample from the adjacent River Wye drainage. The source of the observed genetic variation is discussed in relation to estimated divergence times for Usk mtDNA genotypes and the stocking history of the catchment.