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Evaluation of flow cytometry as a method for quantification of circulating blood cell populations in salmonid fish
Author(s) -
Morgan J. A. W.,
Pottinger T. G.,
Rippon P.
Publication year - 1993
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1993.tb00311.x
Subject(s) - biology , flow cytometry , acridine orange , cell sorting , population , blood cell , microbiology and biotechnology , cytometry , cell , staining , lymphocyte , rainbow trout , fish <actinopterygii> , immunology , fishery , biochemistry , genetics , demography , sociology
Flow cytometry was used to estimate the proportions of different blood cell types in brown and rainbow trout. On the basis of forward light scatter and 90° side scatter three populations were differentiated. The relative abundance of these cells correlated with that of erythrocytc (r 2 = 0.994), lymphocyte plus thrombocyte(r 2 = 0.676) and neutrophil populations (r 2 = 0.571) enumerated by direct microscopy. By density gradient separation of cells, cell sorting and acridine orange staining it was confirmed that these cell types could be assigned to the populations detected. Changes in blood cell populations were monitored by flow cytometry in a group of experimental fish placed under confinement stress. Flow cytometry proved to be a rapid and reliable method for monitoring cell population dynamics in fish blood.