Premium
Biochemical genetic comparison of bighead ( Hypophthalmichthys molitrix ), silver carp ( Aristichthys nobilis ), and their hybrids reared in Czechoslovakia
Author(s) -
Slechtova V.,
Slechta V.,
Hiep Do Doan,
Valenta M.
Publication year - 1991
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1991.tb05097.x
Subject(s) - hypophthalmichthys , bighead carp , biology , hybrid , silver carp , introgression , zoology , grass carp , genetics , gene , botany , fishery , fish <actinopterygii>
Incidental and/or uncontrolled hybridization between silver carp ( Hypophthalmichthys molitrix ) and bighead ( Aristichthys nobilis ) represents a serious problem in Czechoslovak aquaculture. This fact affects fitness traits very negatively in successive generations of hybrids. To solve this problem, 1076 individuals in a total of both H. molitrix. A. nobilis , and their hybrids in 12 groups from six rearing facilities were analysed. Twelve protein systems representing 21 presumptive loci were studied to analyse the electrophoretic patterns of their products using horizontal starch gel electrophoresis of blood and tissue extracts. Both species displayed identical electrophoretic patterns in MYO‐I* , LDH‐A *, LDH‐B *, sMDH‐1 *, sIDHP‐3*, GPI‐1*, and CK‐I * loci. For a reliable differentiation of both species products of the following nine loci are applicable ALB *, PA *, TF, sMDH‐2 *, SOD *, NDH *, MYO‐IF , MYO‐III *, and CK‐2 *. In addition, some polymorphic variants in slDHP‐1 *, sIDHP‐2 *, LDH‐C *, EST‐II *, and GPI‐2 * loci are of use as auxillary markers while the other variants are common to both species. A high level of gene introgression was evident through hybridization between both species. All groups declared previously as ‘ H. molifrix ’ were actually confirmed biochemically to be H. molitrix . However, all groups declared as ‘ A. nobilis ’ were proved to be a mixture of A. nobilis and its hybrids of different level with H. molitrix . This suggests it is impossible to distinguish between A. nobilis and hybrids using their external morphology only.