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Development and use of striped bass‐specific RFLP probes
Author(s) -
Wirgin I. I.,
Maceda L.
Publication year - 1991
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1991.tb05080.x
Subject(s) - biology , bass (fish) , morone saxatilis , restriction enzyme , restriction fragment length polymorphism , morone , genetics , mitochondrial dna , population , nuclear dna , zoology , genotype , evolutionary biology , fishery , dna , gene , demography , sociology
We sought to develop nuclear DNA (nDNA) probes which could be used to complement mtDNA and DNA fingerprinting markers in distinguishing striped bass, Morone saxatilis (Walbaum), from discrete spawning systems. Restriction endonuclease‐generated single copy, 10–20‐kb striped bass nuclear nDNA fragments were cloned into the bacteriophage vector Lambda Dash II and tested in Southern blot analyses for their abilities to reveal population‐specific polymorphisms. Three of the I7 nDNA sequences tested exhibited polymorphisms which potentially could be used to delineate striped bass populations. One probe, DSB 22, revealed significant genotypic frequency differences between Gulf of Mexico and Atlantic striped bass and among striped bass representative of some Atlantic systems. These preliminary results suggest that single copy nDNA sequences may provide sufficient polymorphisms to aid in stock identification of species which proved genetically monomorphic using other approaches.