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Ultrastructural and cytochemical observations on the granulocytes of the sturgeon, Acipenser brevirostrum (Chondrostei)
Author(s) -
Hine P. M.,
Wain J. M.
Publication year - 1988
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1988.tb05466.x
Subject(s) - cytochemistry , ultrastructure , biology , acid phosphatase , esterase , granule (geology) , biochemistry , alkaline phosphatase , granulocyte , microbiology and biotechnology , enzyme , anatomy , immunology , paleontology
Granulocytes from cranial granulopoietic tissue were studied under the electron microscope, and cytochemistry carried out oncranial and peripheral blood granulocytes of two sturgeons, Acipenser brevirostrum . Ultrastructurally, eosinophils and basophils had homogeneous electron‐dense granules similar to those of teleosts and some higher vertebrates. Neutrophils contained two granule types: small elongated fibrillar granules and large (<3.8μm long) usually homogeneous granules. Neutrophil fibrillar granules were positive for alkaline phosphatase (ALP), acid phosphatase (ACP), α‐naphthyl acetate esterase (ANAE), acetyl‐l‐tyrosine‐α‐naphthyl esterase (ATNE) and periodic acid Schiff (PAS) reaction. The large homogeneous granules were negative for all enzymes, and were only PAS positive. Eosinophils had granular, cyanide‐, azide‐ and aminotriazole‐resistant peroxidase (PO) and were ACP, ATNE, tosyl‐l‐lysine‐α‐naphthyl esterase (TLNE) and Luxol fast blue positive. Ultrastructure and cytochemistry are discussed in relation to other vertebrates, and eosinophils identified as the main phagocytic leucocyte.