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An automated method for counting and sizing fish eggs
Author(s) -
Witthames P. R.,
Walker M. Greer
Publication year - 1987
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1987.tb05748.x
Subject(s) - analyser , sizing , biology , range (aeronautics) , histogram , voltage , pulse (music) , fish <actinopterygii> , statistics , materials science , fishery , mathematics , optics , electrical engineering , computer science , physics , artificial intelligence , engineering , art , image (mathematics) , composite material , visual arts
This paper describes an automated method for counting and sizing fish eggs in the diameter range 200–2500 μm. Eggs, suspended in water, are pumped at a controlled rate through an electronic sensor which produces a voltage pulse which is proportional in amplitude to particle size. The sensor is connected to a HIAC Criterion PC‐320 particle analyser which is in turn connected to a Tracor Northern pulse height analyser. This equipment separates the voltage pulses into 398 recording channels. An ACT 1 Sirius microcomputer is used to store the data, transform it to egg size and then produce a histogram of egg size distribution in 20‐μm class intervals. The method has a counting accuracy of 99.5 ± 0.5%. It has been compared with two manual methods of estimating egg numbers.