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Cercariae and diplostomules of Diplostomum spathaceum (Digenea) elicit an immune response in rainbow trout, Salmo gairdneri Richardson
Author(s) -
Whyte S. K.,
Allan J. C.,
Secombes C. J.,
Chappell L. H.
Publication year - 1987
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1987.tb05311.x
Subject(s) - rainbow trout , biology , salmo , digenea , immune system , trematoda , antibody , immunofluorescence , larva , zoology , immunology , helminths , fish <actinopterygii> , fishery , ecology
The immune response of rainbow trout to cercariae and diplostomules of Diplostomum spathaceum was investigated. Following immunization, serum was tested for the presence of antibody, using an enzyme‐linked immunosorbent assay (ELISA) and immunofluorescence. Circulating antibody was detected to both cercariae and diplostomules by 6–7 weeks postimmunization and the sera were tested for their cross‐reactivity with the other larval stage, using an ELISA.