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Preliminary studies on eicosanoid production by fish leucocytes, using GC‐mass spectrometry
Author(s) -
Rowley A. F.,
Barrow S. E.,
Hunt T. C.
Publication year - 1987
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1987.tb05301.x
Subject(s) - eicosanoid , metabolite , biology , thromboxane , prostacyclin , leukotriene , gas chromatography–mass spectrometry , mass spectrometry , ionophore , gas chromatography , chromatography , biochemistry , arachidonic acid , chemistry , platelet , immunology , enzyme , membrane , asthma
Culture supernatants from dogfish leucocytes, exposed to the Ca 2+ ionophore A23187, were analyzed for eicosanoid production by gas chromatography‐electron capture mass spectrometry. Consistently high levels of the prostaglandins (PG) D 2 , F 2α and E 2 were produced, while thromboxane B 2 and leukotriene B 4 were found in smaller amounts. No 6‐oxo PGF 1α , a degradation product of prostacyclin, or 13,14‐dihydro‐15‐oxo‐PGF 2α , a metabolite of PGE 2 and PGF 2α , were detected. The results are compared with similar experiments using mammalian leucocytes.