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Activation of rainbow trout macrophages
Author(s) -
Chung S.,
Secombes C. J.
Publication year - 1987
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1987.tb05292.x
Subject(s) - rainbow trout , biology , in vivo , concanavalin a , in vitro , aeromonas salmonicida , peritoneal cavity , phagocytosis , biochemistry , uridine , phenol red , microbiology and biotechnology , respiratory burst , rna , fish <actinopterygii> , anatomy , fishery , genetics , gene
Rainbow trout peritoneal macrophages were stimulated in vitro using Concanavalin A (Con A) and in vivo using formalin‐killed Aeromonas salmonicida in Freund's incomplete adjuvant (FIA). Whether these cells had been activated was determined by the measurements of oxygen anions (NBT reduction), H 2 O 2 production (oxidation of phenol red), RNA synthesis ( 3 H‐uridine incorporation), acid phosphatase activity and bactericidal activity. In vitro ‐stimulated macrophages showed an increased NBT reduction and 3 H‐uridine incorporation over a range of Con A concentrations, compared with untreated control macrophages, but no detectable increases in H 2 O 2 production or bactericidal activity were observed. On the other hand, in vivo ‐stimulated peritoneal cells showed increases in all the assays compared with FIA‐elicited control cells, and were considered to have been activated.