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Haemolytic activity in the serum of brown trout, Salmo trutta
Author(s) -
Ingram G. A.
Publication year - 1987
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1987.tb05286.x
Subject(s) - hemolysin , biology , trout , brown trout , antibody , lytic cycle , salmo , guinea pig , heterologous , complement system , microbiology and biotechnology , immunology , biochemistry , fish <actinopterygii> , virus , virulence , endocrinology , fishery , gene
Brown trout serum contains a natural, spontaneous, antibody‐independent lytic activity and a haemolysin antibody complement‐mediated lytic activity against unsensitized and trout antibody‐sensitized sheep erythrocytes, respectively. The use of various activators and inactivators of the mammalian complement system demonstrated that trout serum possesses complement or complement‐like components similar in activity to those present in the classical and alternative pathways found in mammals. A single injection of trout with sheep erythrocytes stimulated the production of antibody‐secreting cells in lymphoid organs and increased the levels of natural haemolysins. A second injection of sheep erythrocytes further raised the haemolysin values and antibody‐secreting cell counts. Serum complement from homologous or closely related fish species was more effective for use in the haemolysin and antibody‐secreting cell assays than that from heterologous sources, except guinea pig. Based on physico‐chemical properties, gel filtration and immunoelectrophoretic studies, natural and induced anti‐sheep erythrocyte haemolysins were found to be similar molecules and are possibly high molecular weight IgM antibodies.