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The effects of sodium pentachlorophenate, diet and sampling procedure on amine and tryptophan concentrations in the brain of rainbow trout, Salmo gairdneri Richardson
Author(s) -
Sloley B. D.,
Hickie B. E.,
Dixon D. G.,
Downer R. G. H.,
Martin R. J.
Publication year - 1986
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1986.tb05164.x
Subject(s) - rainbow trout , salmo , tryptophan , biology , sodium , trout , zoology , biogenic amine , medicine , dopamine , endocrinology , chromatography , fish <actinopterygii> , neurotransmitter , chemistry , biochemistry , amino acid , fishery , central nervous system , organic chemistry
The response of rainbow trout (Salmo gairdneri Richardson) to sublethal levels of sodium pentachlorophenate (NaPCP) was determined by measuring brain concentrations of the amines dopamine (DA), norepinephrine (NE), 5‐hydroxytryptamine (5‐HT) and the free amino acid tryptophan (TP) using high performance liquid chromatography with electrochemical detection. Sodium pentachlorophenate had no major effect on the concentrations of the biogenic amines. Mean control concentrations (ng g −1 brain, S.E.) were: DA, 146.2, 9.9; NE, 205.8, 16.6; and 5‐HT, 110.5, 8.8. There was a significant dose‐dependent increase in TP concentration. Fish exposed to 200ng I −1 NaPCP showed a 128% increase in TP level relative to the mean control concentration of 1980 (50) ng g −1 brain. The effects of diets varying in lipid and carbohydrate content, anaesthetization with tricaine methanesulphonate or 2‐phenoxyethanol, and electroshock were also investigated. No differences between treatment groups were detected.