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Physiological salines and the mechanical properties of trout red blood cells
Author(s) -
Hughes G. M.,
Kikuchi Y.,
Barrington J.
Publication year - 1986
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1986.tb04955.x
Subject(s) - rainbow trout , suspension (topology) , red blood cell , biology , saline , filtration (mathematics) , red cell , trout , volume (thermodynamics) , suspension culture , zoology , chromatography , medicine , biochemistry , endocrinology , cell culture , chemistry , fish <actinopterygii> , fishery , physics , mathematics , quantum mechanics , statistics , homotopy , pure mathematics , genetics
Suspensions of rainbow trout erythrocytes in different physiological salines were compared with respect to their haematological and filtration properties. A method is described for the suspension of erythrocytes in Cortland saline which has proved suitable for studies of their mechanical properties over periods of several hours. Significant differences were found between whole blood samples taken during cannulation and after several days recovery, particularly mean cell volume, frequency distribution of red cell volumes and the pore passage time through nucleopore filters. These differences were also found using red cell suspensions of the same bloods. The pore passage time of whole blood sampled during cannulation or its suspensions is less than that of recovery blood although its mean cell volume is greater.