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Purification of carp putative eosinophils on metrizamide gradients
Author(s) -
Hendrick M.,
DiNapoli A.,
Cammarata P.,
Pincus S.
Publication year - 1986
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1986.tb04925.x
Subject(s) - metrizamide , biology , carp , differential centrifugation , eosinophil , centrifugation , fish <actinopterygii> , in vitro , buoyant density , immunocytochemistry , density gradient , anatomy , immunology , microbiology and biotechnology , biochemistry , endocrinology , fishery , spinal cord , virus , physics , myelography , quantum mechanics , neuroscience , asthma
A protocol for the purification of carp eosinophils on metrizamide gradients is presented. Density gradient centrifugation is a new approach to the study of eosinophils in fish. It provides enriched populations of viable eosinophils necessary for in vitro functional assays. Eosinophils purified from the peripheral blood of carp resemble mammalian eosinophils by light and electron microscopy. They are indistinguishable from carp neutrophils which have a different density and morphology.