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Preliminary investigation of the characteristics of fish lymphocytes separated on a Percoll discontinuous gradient
Author(s) -
Blaxhall P. C.,
Sheard P. R.
Publication year - 1985
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1985.tb04258.x
Subject(s) - percoll , biology , lymphocyte , polyclonal antibodies , density gradient , microbiology and biotechnology , immunology , antigen , biochemistry , centrifugation , physics , quantum mechanics
This investigation was carried out to determine if lymphocyte functional heterogeneity could be demonstrated in teleosts on the basis of density difference. The lymphocytes separated into two fractions on a Percoll discontinuous gradient. The less dense lymphocytes separated at 1.056gml −1 and contained both moderately villous and smooth lymphocytes, whereas the more dense lymphocytes separated at 1.07 g ml −1 and were predominantly moderately villous lymphocytes. The moderately villous lymphocytes had a higher mitochondria content than the smooth lymphocytes. Lymphocytes separating in the 1.056g ml −1 layer were more responsive to stimulation with polyclonal mitogen (PHA) than the lymphocytes separating in the 1.07 g ml −1 layer. This indicates that the lymphocytes separated according to either cell lineage or maturity.