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Cry (preservation of spermatozoa of the whitefish Coregonus muksun Pallas
Author(s) -
And J. Plironen,
Hyvärinen H.
Publication year - 1983
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1983.tb04736.x
Subject(s) - extender , cryoprotectant , biology , cryopreservation , human fertilization , sperm , semen , glycerol , zoology , andrology , fishery , botany , anatomy , chemistry , biochemistry , embryo , medicine , organic chemistry , polyurethane
The cryopreservation methods for whitefish, Coregonus muksun , spermatozoa were studied using six different extenders with ‐40°C and ‐196°C as storing temperatures. In 1980, the spermatozoa of individual fishes were frozen in dry ice, in vials containing three different media based on glycerol as cryoprotectant. The pure glycerol media resulted in a 20% average rate of fertilization when samples were stored at — 40°C. The thawing procedure did not affect fertilization. In 1981, the sperm of individual fishes was frozen in pellets and stored in liquid nitrogen. Cryopreserved semen thawed in 0·119M NaHCO 3 produced 29·6–87·7% eyed eggs. The differences were caused mainly by the type of extender. The highest rate of fertility was obtained using an extender consisting of 0·3 M glucose with 20% glycerol (87·7% eyed eggs; 98·6% eyed eggs when compared to the corresponding control). The same values, using the modified extender of Stoss (Stoss & Refstie, in press), were 38·7–82·4% and, on the average, 90% eyed eggs, respectively.