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Factors affecting lymphocyte culture for chromosome studies
Author(s) -
Blaxhall P. C.
Publication year - 1983
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1983.tb04726.x
Subject(s) - biology , salmo , cyprinus , karyotype , brown trout , carp , mitosis , chromosome , lymphocyte , transformation (genetics) , trout , zoology , immunology , genetics , fishery , fish <actinopterygii> , gene
Factors affecting the transformation of brown trout, Salmo trutta L. , and carp, Cyprinus carpio L. , lymphocytes in culture for subsequent karyotyping are examined. Optimum conditions for mitosis are established in respect of medium, mitogen, atmosphere, buffering system and serum.