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Bioassay of insulin activity of pancreatic tissue in active and aestivated African lungfish, Protopterus annectens (Owen)
Author(s) -
Hakeem O. H.,
Babiker M. M.
Publication year - 1983
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1983.tb02905.x
Subject(s) - lungfish , endocrinology , medicine , biology , glycogen , insulin , plasma glucose , fishery , fish <actinopterygii>
The insulin activity of the pancreatic tissues of free‐swimming and aestivated African lungfish, P. annectens (Owen), was bioassayed against standard insulin in rabbits. The hepatic glycogen content and plasma glucose concentration in free‐swimming and aestivated lungfish were also estimated. The liver glycogen content was 64·47 ± 6·32 and 7·19 ± 2·68 mgg −1 net wt and the plasma glucose concentration was 37·62 ± 4·22 and 6·39 ± 1·29% in free‐swimming and aestivated lungfish, respectively. Extracts of pancreatic tissue of the lungfish produced a dose‐dependent decrease in the plasma glucose concentration in rabbits. The magnitude of the decrease in plasma glucose concentration produced by extracts from aestivated specimens was significantly smaller (about 26%) compared to that produced by pancreatic extracts from free‐swimming active lungfish (about 37%). The insulin activity of the pancreatic tissue was 0·82 ± 0·02 and 1·27 ± 0·25 iu mg −1 dry pancreatic tissue weight in aestivated and free‐swimming lungfish, respectively.