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Haematological assessment of generally used freshwater fish blood anticoagulants
Author(s) -
Smit G. L.,
Hattingh J.
Publication year - 1980
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1980.tb02768.x
Subject(s) - biology , heparin , oxalate , anticoagulant , preservative , trisodium citrate , biochemistry , food science , medicine , chemistry , inorganic chemistry
Heparin injection B.P., containing 0·5% phenol as preservative, disodium ethylenediamine tetra‐acetate (EDTA), trisodium citrate (TSC) and ammonium potassium oxalate (APO) were compared as anticoagulants for routine haematological investigations in Cyprinus carpio and Sarotherodon mossambicus . The acidic nature and haemolytic effects of EDTA and APO render them unsuitable for use with fish blood. TSC was required in higher concentrations (than heparin) for effective anticoagulation and this was undesirable for the determination of blood pH and pCO2 and certain other parameters. Several important interspecies differences in the reactions towards the anticoagulants are also reported. In general, heparin proved to be the anticoagulant of choice, although unsuitable for some assays.