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Restoration of glycogen from lactic acid in the anaerobic swimming muscle of plaice, Pleuronectes platessa L.
Author(s) -
Batty R. S.,
Wardle C. S.
Publication year - 1979
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1979.tb03641.x
Subject(s) - pleuronectes , glycogen , anaerobic exercise , biology , lactic acid , glycolysis , glycogenesis , gluconeogenesis , medicine , biochemistry , endocrinology , metabolism , fish <actinopterygii> , fishery , glycogen synthase , bacteria , physiology , genetics
The conclusion from two in vivo experiments is that a significant proportion of the lactic acid, normally formed by glycolysis from glycogen and held in the muscle cells following exhausting exercise of the anaerobic swimming muscle of the teleost fish Pleuronectes platessa L, is converted by gluconeogenesis to form glycogen in the recovering muscle. In the first experiment a technique for measurement of [ 3 H]glucose turnover in the plaice was developed and applied to measure turnover in resting and exhausted fish. It is concluded that insufficient glucose was moved through the circulation to account for the rate of glycogen formation observed in the recovering exhausted muscle. In the second experiment, an intramuscular injection of [ 14 C]lactate to exhausted fish revealed a direct uptake of [ 14 C]lactate by the recovering muscle cells, and the incorporation of substantial proportions of lactate into the restored glycogen. Simultaneous use of [ 3 H]‐mannitol allowed measurement of the isotope distribution between extra‐ and intracellular spaces.