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Variability and stability of selected components in rainbow trout Salmo gairdneri serum and the precision of automated analysis in measuring these components *
Author(s) -
Warner M. C.,
Tomb A. M.,
Diehl Sally A.
Publication year - 1979
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1979.tb03577.x
Subject(s) - rainbow trout , biology , salmo , creatinine , creatine kinase , blood urea nitrogen , medicine , albumin , brown trout , blood serum , endocrinology , biochemistry , fishery , fish <actinopterygii>
Eighteen components in rainbow trout serum were tested for variability among individuals and stability during storage. In addition, the precision of an automated serum analysis system was determined. Stability of serum components was observed over 42 days at temperatures of 25° C, 4° C and ‐ 10° C. Components tested included: albumin, total protein, blood urea nitrogen, cholesterol, chloride, glucose, potassium, sodium, cholinesterase, alkaline phosphatase, lactic dehydrogenase, a‐hydroxybutyrate dehydrogenase, glutamic pyruvic transaminase, phosphohexose isomerase, inorganic phosphorus, calcium, creatinine, and creatine phosphokinase. Fish serum was generally more stable than human serum when stored at 25° C and 4° C and similar in stability at ‐ 10° C. Precision of analytical methodologies was excellent for all components measured except creatine phosphokinase.