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T and B cell analogues from peripheral blood of immune channel catfish, Ictalurus punctatus
Author(s) -
Lewis D. H.,
Eurell T. E.,
Can M. S.,
Grumbles L. C.
Publication year - 1979
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1979.tb03492.x
Subject(s) - ictalurus , biology , catfish , lymphocyte , immune system , aeromonas hydrophila , immunology , receptor , microbiology and biotechnology , fish <actinopterygii> , biochemistry , fishery
Immune channel catfish lymphocytes were recovered from peripheral blood on an isotonic (250 mOsm) separation medium consisting of Ficoll‐Hypaque adjusted to a specific gravity of 1·065–1·070 g ml −1 . At least two types of cells similar to T and B lymphocytes of higher vertebrates were observed. One type of lymphocyte, referred to as “small lymphocyte” possessed surface erythrocyte receptors and appeared to be analogous to mammalian T cells. A second type of lymphocyte analogous to mammalian B cell possessed surface immunoglobulins and was capable of cytoadherence with Aeromonas hydrophila cells.