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Observations on development and morphological effects of histamine liberator 48/80 on PAS‐positive granular leukocytes and heterophils of Catostomus commersoni
Author(s) -
Barber D. L.,
Westermann J. E. Mills
Publication year - 1978
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1978.tb03469.x
Subject(s) - metachromasia , histamine , biology , granule (geology) , mast cell , fish <actinopterygii> , immunology , anatomy , endocrinology , fishery , staining , paleontology , genetics
Developmental stages of the two circulating blood granulocytes, the heterophil and the periodic acid‐Schiff‐positive granular leukocyte (PAS‐GL) are described. There are no metachromatic basophils in this fish and eosinophils, when present, are confined to extra‐vascular tissues. Fish were killed up to 24 h after injection with histamine liberator 48/80. Morphological (L/M and E/M) and histochemical studies of blood, haemopoietic tissue and gills from experimental fish were compared with those of untreated control fish. Blood cells other than PAS‐GL and heterophils were generally unaffected by 48/80. Most PAS‐GL and heterophils showed alterations in granules and other cytoplasmic features. Stretching and splitting of the PAS‐GL nucleus occurred. Very little, if any, histamine was detectable in PAS‐GL and heterophils. The authors support the postulate that during evolution PAS‐GL become basophils/ mast cells through two major stages, (1) sulphation of the granule polysaccharide to give heparin resulting in metachromasia of the granules and (2) storage of large quantities of histamine in the granules.