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Control of whirling disease (Myxosoma cerebralis): use of methylene blue staining as a possible indicator of effect of heat on spores
Author(s) -
Hoffman Glenn L.,
Markiw Maria E.
Publication year - 1977
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1977.tb04051.x
Subject(s) - methylene blue , spore , staining , biology , microbiology and biotechnology , biochemistry , photocatalysis , catalysis , genetics
Methylene blue staining (0.08 %) was used to determine efficiency of heat treatment in killing spores of Myxosoma cerebralis . Nearly all spores heated at 90°C for 10 min and 70°C for 100 min became stained giving presumptive evidence that they were killed.