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The immunohistochemical localization of lysozyme in plaice (Pleuronectes platessa L.) tissues
Author(s) -
Murray C. K.,
Fletcher T. C.
Publication year - 1976
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1976.tb04681.x
Subject(s) - pleuronectes , lysozyme , biology , mucus , antiserum , immunodiffusion , radial immunodiffusion , spleen , antibody , anatomy , pathology , microbiology and biotechnology , fish <actinopterygii> , fishery , biochemistry , immunology , ecology , medicine
The location of lysozyme in fresh plaice ( Pleuronectes platessa L.) tissues was studied using an immunofluorescent technique. An antiserum to a homogeneous sample of plaice serum lysozyme was prepared in rabbits and was shown to be monospecific by immunodiffusion and inhibition studies. This preparation, used in conjunction with fluorescent labelled sheep anti‐rabbit immunoglobulin, demonstrated lysozyme in plaice neutrophils, peritoneal macrophages, gill cartilage and epithelium, spleen, kidney, skin and mucus. The distribution of lysozyme in the skin and secreted mucus was affected by the method of killing the fish.