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Induced polyploidy in Tilapia aurea (Steindachner) by means of temperature shock treatment
Author(s) -
Valenti R. J.
Publication year - 1975
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1975.tb04627.x
Subject(s) - biology , tilapia , ploidy , insemination , sperm , polyploid , incubation , zoology , cobitidae , fish <actinopterygii> , andrology , botany , toxicology , fishery , genetics , biochemistry , medicine , gene
A technique for increasing ploidy in the blue tilapia, Tilapia aurea , was investigated for the purpose of determining its effect on standard length. Gametes of T. aurea were stripped from ripe adults and eggs were fertilized with donor sperm from several males. A description of the extra‐oral incubation system used for the embryos is provided. This system resulted in a maximum of 90% successful hatch. Normal egg development was interrupted by immersion of newly fertilized eggs (15 min post‐insemination) in cold or hot water for various durations. The reliability of these techniques for inducing polyploidy was examined using increased volume of erythrocyte nuclei as a criteria for success. A cold shock of from 32°C (ambient) to 11°C for a 1 h duration resulted in the largest incidence of polyploidy (75 %). Polyploid fish were found to be larger than diploid siblings when measured at 14 weeks of age.