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Histochemical analysis of mucous cells in the epidermis of brown trout Salmo trutta L.
Author(s) -
Harris J. E.,
Watson Aileen,
Hunt S.
Publication year - 1973
Publication title -
journal of fish biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.672
H-Index - 115
eISSN - 1095-8649
pISSN - 0022-1112
DOI - 10.1111/j.1095-8649.1973.tb04463.x
Subject(s) - brown trout , salmo , biology , mucus , mucin , epidermis (zoology) , neuraminidase , sialic acid , periodic acid–schiff stain , trout , fish <actinopterygii> , biochemistry , anatomy , immunohistochemistry , enzyme , fishery , immunology , ecology
A histochemical study of the epidermal mucous cells of brown trout revealed that they contained both neutral and acidic mucosubstances which were diastase‐resistant, PAS‐reactive. Neuraminidase treatment, methylation and combined staining procedures suggested that the acidic nature of the mucins was due mainly to the presence of sialic‐acid containing glycoprotein. These results augment data derived from biochemical analyses of the epidermal mucus of salmonid fish.