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Identification of a Novel Mutation Affecting Domain V of the 23S rRNA Gene in Helicobacter pylori
Author(s) -
Toracchio Sonia,
Aceto Gitana M.,
MarianiCostantini Renato,
Battista Pasquale,
Marzio Leonardo
Publication year - 2004
Publication title -
helicobacter
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 79
eISSN - 1523-5378
pISSN - 1083-4389
DOI - 10.1111/j.1083-4389.2004.00267.x
Subject(s) - 23s ribosomal rna , clarithromycin , helicobacter pylori , transversion , mutant , microbiology and biotechnology , biology , mutation , gene , ribosomal rna , gene mutation , genetics , rna , ribosome
Background. This study analyzes clarithromycin resistance status and 23S rRNA gene mutations in Helicobacter pylori strains from Central Italian patients. Materials and methods. H. pylori strains from 235 dyspeptic patients (205 with no history of clarithromycin exposure and 30 referred for failure of eradication therapy) were tested for clarithromycin resistance by screening agar method and E ‐test. Resistant strains were analyzed for mutations of the 23S rRNA gene by PCR‐RFLP and sequencing. Results. Primary resistance was observed in strains from 43/205 (21%) patients with no history of clarithromycin exposure and secondary resistance in 30/30 (100%) strains from previously treated patients. A single mutant strain was detected in 54/73 (74%) cases, a mixture of one or more mutant(s) plus the wild type in the remaining 19/73 (26%) cases. One 23S rRNA gene mutation (A→T transversion at nucleotide 2144) in the peptidyltransferase region of domain V was novel. Conclusions. This study shows: (a) a high prevalence of H. pylori strains with primary or secondary clarithromycin resistance in an urban area of Central Italy; (b) colonization by both mutant and wild‐type H. pylori in the same patient; (c) a novel variant of the H. pylori 23S rRNA gene.