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ß2‐adrenergic receptor activation delays dermal fibroblast‐mediated contraction of collagen gels via a cAMP‐dependent mechanism
Author(s) -
Pullar Christine E.,
Isseroff R. Rivkah
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130408.x
Subject(s) - contraction (grammar) , fibroblast , wound healing , agonist , dermal fibroblast , chemistry , extracellular , microbiology and biotechnology , cyclic adenosine monophosphate , receptor , keratinocyte , pharmacology , in vitro , endocrinology , medicine , biochemistry , biology , immunology
Dermal fibroblasts actively contribute to wound healing by migrating to the wound, synthesizing extracellular matrices, and generating mechanical forces within the wound to initiate wound contraction. Fibroblast‐seeded collagen gels provide an in vitro model to study wound contraction. The authors are evaluating the role of the adrenergic signaling system in cutaneous wound repair and recently found that β2‐adrenergic receptor (β2‐AR) activation markedly decreases keratinocyte migration, an essential step in wound reepithelialization. Because the β2‐ARs are also expressed on dermal fibroblasts, a study was initiated to determine the effects of β‐adrenergic agonists on dermal fibroblast‐mediated collagen gel contraction. A β‐agonist (isoproterenol) delayed gel contraction in a dose‐dependent manner. A β2‐AR specific antagonist (ICI 118,551) prevented the delay, indicating that the β2‐AR alone mediated the delay. The active cyclic adenosine monophosphate (cAMP) analog also delayed collagen gel contraction, whereas an inactive cAMP analog partially prevented the delay, suggesting that the mechanism for β‐AR agonist–mediated delay was partly cAMP‐dependent. Identifying and characterizing agents that modulate wound contraction improves understanding of the wound healing process and could result in novel therapeutic strategies for preventing unwanted wound contraction in burn and trauma patients.