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Acceleration of wound healing in healing‐impaired db/db mice with a photocrosslinkable chitosan hydrogel containing fibroblast growth factor‐2
Author(s) -
Obara Kiyohaya,
Ishihara Masayuki,
Fujita Masanori,
Kanatani Yasuhiro,
Hattori Hidemi,
Matsui Takemi,
Takase Bonpei,
Ozeki Yuichi,
Nakamura Shingo,
Ishizuka Takamitsu,
Tominaga Susumu,
Hiroi Sadayuki,
Kawai Toshiaki,
Maehara Tadaaki
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130406.x
Subject(s) - chitosan , wound healing , fibroblast , biomedical engineering , in vivo , self healing hydrogels , contraction (grammar) , chemistry , fibroblast growth factor , materials science , biophysics , surgery , medicine , in vitro , polymer chemistry , endocrinology , biochemistry , biology , receptor , microbiology and biotechnology
Application of ultraviolet light irradiation to a photocrosslinkable chitosan (Az‐CH‐LA) aqueous solution including fibroblast growth factor‐2 (FGF‐2) results within 30 seconds in an insoluble, flexible hydrogel. The FGF‐2 molecules retained in the chitosan hydrogel remain biologically active and are released from the chitosan hydrogel upon in vivo biodegradation of the hydrogel. To evaluate the accelerating effect on wound healing of this hydrogel, full‐thickness skin incisions were made in the backs of healing‐impaired diabetic ( db/db ) mice and their normal ( db/+) littermates. The mice were later killed, and histological sections of the wound were prepared. The degree of wound healing was evaluated using several histological parameters such as the rate of contraction, epithelialization, and tissue filling. Application of the chitosan hydrogel significantly advanced the rate of contraction on Days 0 to 2 in db/db and db/+ mice. Although the addition of FGF‐2 into the chitosan hydrogel in db/+ mice had little effect, application of the chitosan hydrogel–containing FGF‐2 further accelerated the adjusted tissue filling rate (Days 2 to 4 and Days 4 to 8) in db/db mice. Furthermore, the chitosan hydrogel–containing FGF‐2 markedly increased the number of CD‐34‐positive vessels in the wound areas of db/db mice on Day 4. Thus, the application of chitosan hydrogel–containing FGF‐2 onto a healing‐impaired wound induces significant wound contraction and accelerates wound closure and healing.