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Correlation of Acute Human and Murine Wound Healing with and without Topical Growth Factor Therapy
Author(s) -
Li W.W.,
Li V.W.
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130216r.x
Subject(s) - wound healing , angiogenesis , medicine , growth factor , platelet derived growth factor receptor , immunohistochemistry , vascular endothelial growth factor , platelet derived growth factor , pathology , gene expression , biopsy , cancer research , gene , surgery , vegf receptors , biology , receptor , biochemistry
Background: Previously, we showed in a human forearm biopsy model of wound healing that acute healing dynamics can be characterized at the clinical and genetic level. Growth factor application by recombinant human platelet‐derived growth factor‐BB (rhPDGF‐BB) accelerates the velocity of wound healing and modulates gene expression in wounds. To profile the function, structure, and genomics of acute healing, the human results were compared with a murine model of acute circular and incisional wounds. Methods: Full thickness 8 mm circular skin wounds and linear surgical incisional wounds were created in 6‐week‐old Balb‐C mice and recombinant angiogenic growth factors (PDGF vs. PDGF/FGF2/VEGF) were administered topically to wounds daily. Velocity of healing and time to complete closure were determined by digital planimetry. Tissue was examined for histopathological, morphometric, functional, and gene expression parameters, and compared to human results. Results: Acute healing velocity can be quantified and compared in human and murine models. Linear incisions heal in distinct morphological, immunohistochemical and gene expression patterns compared to circular wounds. The cellular profile shows differences between wound types. Topical PDGF accelerates angiogenesis and time to complete acute healing. Human and murine gene expression was correlated. Results will be presented. Acknowledgments: Supported by a grant from The Angiogenesis Foundation