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The influence of inhibition of postburn stress on wound sepsis in severely scalded rats
Author(s) -
Liang Qiao,
Huizhong Yang,
Kejian Yuan,
Heliang Dong,
Wenkui Wang,
Weishi Xu
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130216bo.x
Subject(s) - resuscitation , sepsis , medicine , cytokine , messenger rna , immune system , anesthesia , immunology , andrology , biology , biochemistry , gene
Objective: To investigate the effects of hibernation drugs on early wound sepsis in severely scalded rats. Methods: Sprague‐Dawley rats inflicted 30% TBSA deep thickness were randomly divided into A (immediate resuscitation) and B (immediate resuscitation and lytic cocktail). After infusion of 10 8 CFU/ml Pseudomonas aeruginosa in the wound on third postburn day, survival was compared between two groups. Local tissue samples and plasma were collected and the plasma level of IL‐2 mRNA, IL‐4 mRNA, IL‐10 mRNA, and IFN‐γ mRNA were assayed by fluorescence PCR. Results: Compared with Group A, host survival significantly increased (0.663 ± 0.026, P < 0.01), viscera injury decrease in group B after infusion bacteria. The plasma Th1 cytokine mRNA (IL‐2, IFN‐γ) levels in B group were significantly increased compared with A group. However, the plasma Th2 cytokine mRNA (IL‐4, IL‐10) levels in group B were not as significantly increased as group A. Conclusions: Immediate resuscitation with hibernation drugs can decrease viscera injury after sepsis, increase host survival and improve host immune system by preventing Th1 cell from over activation and decrease Th2 cytokines.