Premium
148
In Vivo Adenoviral Gene Transfer of SPARC in a Skin‐Humanized Mouse Wound Healing Model
Author(s) -
Escámez M.J.,
Carretero M.,
Prada F.,
Larcher F.,
García M.,
Mirones I.,
Holguín A.,
Duarte B.,
Jorcano J.L,
Podhajcer O.,
Del Río M.
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130216az.x
Subject(s) - wound healing , in vivo , extracellular matrix , regeneration (biology) , matricellular protein , gene transfer , transplantation , microbiology and biotechnology , human skin , pathology , cancer research , chemistry , medicine , biology , immunology , gene , surgery , biochemistry , genetics
SPARC (secreted protein, acidic and rich in cysteine), a matricellular glycoprotein, modulates the interactions of cells with the extracellular matrix. Studies in null‐mice revealed a role of SPARC in wound healing. Here we examined the effect of SPARC in a skin‐humanized mouse wound healing model. This model is based in the regeneration of human skin onto the back of nude mice by transplantation of a dermo‐epidermal equivalent. The regenerated human skin was excisionally wounded with biopsy punches. At the moment of wounding an adenoviral vector encoding the cDNA for SPARC was intradermally injected. We are currently assessing the effects of in vivo gene transfer of SPARC at the wound site in the healing process. Criticals events of wound healing including reepithelialization, regeneration of dermoepidermal junction and dermal remodeling were studied at different time points postwounding.