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Retaining the Viability of Human Amniotic Membrane for Use in Burn Wound Coverage
Author(s) -
Hennerbichler S.,
Reichl B.,
Gabriel C.,
Eibl J.,
Redl H.
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130216am.x
Subject(s) - membrane , glycerol , viability assay , andrology , amnion , wound healing , amniotic fluid , chemistry , cell , medicine , surgery , biology , fetus , biochemistry , pregnancy , genetics
Currently freeze‐dried, gamma‐sterilized, or glycerol‐preserved amniotic membranes are widely used. However, it is not clear whether this devitalized state is the optimal application form. Therefore within this study the ideal condition for midterm storage of human amniotic membranes was assessed to ensure the availability of vital amniotic membranes, in particular for burn wounds. Methods and Materials: For this purpose mothers were serologically tested and term placentae were collected and washed. After the amniotic membrane was peeled off and further washed, biopsies were taken for microbiological testing and various storage experiments (different media and temperatures).• cell culture medium, 37 °C • glycerol, 4 °C • 10% DMSO, −80 °CViability of fresh and stored amniotic membranes was determined with the MTT‐based EZ4U‐ Assay (Biomedica, Vienna, Austria). Results and Discussion: Best results were obtained while storing the membranes in cell culture medium at 37 °C, whereas storage in glycerol at 4 °C resulted in cell death within the first 4 days. To our knowledge this is the first study investigating the viability of amniotic membrane under different storage conditions. The influence on wound healing is currently under investigation.