Premium
092
Natural Extracellular Matrix Improves Wound Repair Measures
Author(s) -
Hiles M.C.,
Hodde J.P.,
Suckow M.A.
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130215cn.x
Subject(s) - wound healing , medicine , trichrome , contracture , masson's trichrome stain , extracellular matrix , submucosa , surgery , pathology , staining , biology , h&e stain , microbiology and biotechnology
Small intestinal submucosa (SIS) has found substantial clinical utility as a commercially available wound matrix. In the present study, the utility of two natural, nonsynthetic and noncrosslinked ECMs, porcine renal capsule material (RCM) and SIS, were compared to no‐fill controls in a rat full thickness skin wound model. Groups of rats had surgically created wounds filled with SIS, RCM, or left unfilled (ten/treatment/time point). Wound diameters periodically measured at 3–30 days after wound creation and sites sampled at 3–56 days after wound creation were numerically graded for degree of histologic change including the formation of adnexa and for collagen content based on intensity of trichrome staining. Wounds in both ECM groups had statistically significant (P < 0.001) greater wound diameters than unfilled controls at days 7, 12, and 17 with no differences between RCM‐ and SIS‐treated wounds at any time point. At 56 days, SIS‐filled and RCM‐filled wounds were completely closed with cornified epithelium covered by a keratinized layer. Healing was largely complete, with normal numbers of adnexal structures present in most sections, while unfilled control wounds were similar though fewer adnexal structures were present. Though histology scores suggested more robust healing with either RCM or SIS, no significant differences with unfilled control wounds were found. From this study, it appears that RCM and SIS enhance wound healing in terms of wound tissue collagenization and maturation of adnexal structures while clearly impeding wound contraction. Natural ECMs have utility for dermal wound repair and may lessen clinical contracture formation caused by rapid wound contraction. Acknowledgment: This work was supported by Cook Biotech Incorporated.