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Connective Tissue Growth Factor (CTGF) Content and Bioactivity of Small Intestinal Submucosa (SIS)
Author(s) -
Janis A.D.,
Klingman N.V.,
Berceli S.A.
Publication year - 2008
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130215bs.x
Subject(s) - ctgf , connective tissue , extracellular matrix , growth factor , chemistry , wound healing , biomaterial , biomedical engineering , pathology , microbiology and biotechnology , immunology , medicine , biochemistry , biology , receptor
Bioactive, resorbable materials can be useful in the treatment of surgical injury where biomaterial incorporation followed by tissue‐specific remodeling is desired. Bioactive growth factors may be useful in the treatment of chronic ulcerative wounds, shifting the static wound environment to a more active acute phase. Small intestinal submucosa (SIS) is a naturally occurring extracellular matrix used as a biomaterial for the treatment of soft tissue injury, as well as pressure, venous, and diabetic ulcers. Preclinical and clinical studies have demonstrated that SIS induces host tissue infiltration, site‐specific remodeling, and repair. Although SIS consists primarily of collagen, the presence of other proteins, glycosaminoglycans, proteoglycans, and the growth factors FGF‐2, VEGF, and TGF‐β have been reported. Recent experiments have demonstrated the presence of connective tissue growth factor (CTGF) in SIS. Immunohistochemical staining for CTGF was positive in mechanically isolated, disinfected, and ethylene oxide (EO) sterilized SIS. Lyophilized SIS samples were ground in ice cold PBS and CTGF concentration was measured using ELISA. The results are summarized in the following table:Sample [CTGF](ng/g)Mechanically isolated SIS 39.88 ± 5.4 Disinfected SIS 11.31 ± 7.2 Ethylene oxide sterilized SIS 7.96 ± 1.3CTGF, secreted by fibroblasts after activation with TGF‐β, stimulates cell proliferation and extracellular matrix synthesis. In vitro cell culture bioactivity experiments are planned. This work was supported by Cook Biotech Incorporated.