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Dressing Effects on Cellular Proliferation and Assessment of Fibroblast's Adherence in Vitro
Author(s) -
Bernard FX,
Barrault C
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130117c.x
Subject(s) - extracellular matrix , fibroblast , in vitro , cell growth , chemistry , staining , microbiology and biotechnology , confocal , myofibroblast , wound healing , cell , confocal microscopy , mtt assay , ultrastructure , matrix (chemical analysis) , pathology , biology , immunology , medicine , biochemistry , chromatography , fibrosis , geometry , mathematics
The aim of this study was to compare the effects of Urgotul ® and other greasy dressings and interfaces on normal human dermal fibroblasts (NHDF) monolayers in vitro . The selected end points were the cell proliferation, the morphology of the extracellular matrix (ECM) upon dressing removal, and the structure of the underlying fibroblasts. Materials and methods: Equivalent pieces of each dressing were applied on NHDF monolayers for different times. Cell proliferation was measured via [3H] thymidine incorporation. Identical cultures were used to assess the dressing impact on the morphology of cells in culture after MTT staining and micro‐photographing. ECM morphology was shown by immunoflorescence, using an anticollagen I antibody. Effects on cell ultrastructure were documented by confocal laser microscopy after tubulin/actin double labelling. Results: Among the five tested greasy dressings and interfaces, only Urgotul ® showed a stimulating effect on the proliferation of NHDF. Two dressings did not modify proliferation and two other had cytostatic effects. In addition, the lesions of the ECM upon dressing removal were clearly the lowest with Urgotul ® (low adherence to cellular surface and/or to extracellular matrix). The ultrastructure of the NHDF in direct contact with Urgotul ® was not significantly modified. Conclusion: Fibroblasts are the key cells of wound healing. The use of nonaggressive greasy gauzes and/or interfaces promoting cell proliferation should be of interest. Clinical evaluations are required to confirm these in vitro results.