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General session V
Moderated by Yashihiko Maehara: Reduction of Ischemia‐Reperfusion Injury to Skin Flaps by Monoclonal Antibody to Leukocyte Endothelial Adhesion Molecule‐1
Author(s) -
Tosa Yasuyoshi,
Muramatsu H.,
Hukushiyama H.,
Otsuka T.,
Satoh K.,
Hosaka Y.
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130116s.x
Subject(s) - ischemia , medicine , reperfusion injury , cell adhesion molecule , monoclonal antibody , surgery , endothelium , adhesion , saline , pathology , andrology , immunology , antibody , chemistry , organic chemistry
Aim: Ischemia‐reperfusion (I‐R) injury continues to be a problem for successful free tissue transfer and replantation after prolonged periods of ischemia. It has been shown that leukocytes and vascular endothelial cells release a variety of inflammatory mediators during reperfusion after ischemia. The leukocyte endothelial adhesion molecule‐1 (LECAM‐1) is a ligand on the endothelium for some of the adhesion receptors on leukocytes. The purpose of this study was to evaluate the blockage of leukocyte‐endothelial adhesion by a monoclonal antibody (MAb) to LECAM‐1 in skin flaps to prevent I‐R injury in rats. Methods: Male SD rats (225–250 g) were used. A skin flap (45 × 30 mm) supplied by the superficial epigastric A&V including the femoral vessels was isolated unilaterally. The femoral vessels were cross‐clamped the epigastric vessels for 9 hours of ischemia. Animals in treated group received MAb to LECAM −1 i.v. 15 minutes prior to reperfusion; those in the control group received normal saline. Skin flap viability was assessed by tracing the outline of viable and nonviable areas. Data were collected for the following 7 days. These data were corroborated with histological evidence on comparable areas of the flap. Results: Tracing analysis revealed average flap survival area of about 90% in treated group and about 20% in control group (p < 0.05). Histopathologically, few inflammatory changes could be observed in treated group, while marked damage was observed in control group. Conclusions: From this study, we concluded that treating skin flaps with Mab to LECAM ‐1 was effective for I‐R injury after 9 hours of warm ischemia.