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Comparison of Bacteria‐Retaining Ability of Wound Dressings
Author(s) -
Tachi Masahiro,
Hirabayashi Shinichi,
Yonehara Yoshiyuki,
Uchida Gentaro,
Tohyama Takuya
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130116r.x
Subject(s) - staphylococcus aureus , pseudomonas aeruginosa , wound dressing , saline , bacterial colony , wound healing , colony forming unit , microbiology and biotechnology , bacteria , medicine , chemistry , surgery , biology , anesthesia , materials science , genetics , composite material
We studied the bacterial retention capacity of alginate and carboxymethylcellulose dressings, using an infected skin ulcer model on the backs of rats. Wound surfaces were inoculated with either Staphylococcus aureus or Pseudomonas aeruginosa at a concentration of 1.5 × 10 6 cfu/wound. Carboxymethylcellulose dressing, alginate dressing A and alginate dressing B were applied to the contaminated wounds for 12 hours. Each dressing was then divided into two piece. Total viable bacterial count within the dressing was calculated using one piece, and bacterial count released from the dressing into physiological saline was determined using the other piece, enabling bacterial retention rate to be calculated. Each dressing was tested on each of 10 wounds. Statistical analyses were performed using one‐way analysis of variance (ANOVA) for replicated measures combined with Duncan's Multiple Comparison Test. Carboxymethylcellulose dressing was most effective in its ability to retain both Staphylococcus aureus and Pseudomonas aeruginosa (p < 0.05). Conclusions: The bacterial retaining ability of carboxymethylcellulose dressing was found to be significantly higher than that of alginate dressings in an infected animal wound model.