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Gene Expression of Liver Regeneration After Partial Hepatectomy in Mice Lacking Type Tumor Necrosis Factor Receptor
Author(s) -
Shimizu Tetsuya,
Togo Shinji,
Makino Hirochika,
Tanaka Kuniya,
Nagasima Yoji,
Shimada Hiroshi
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130116e.x
Subject(s) - liver regeneration , tumor necrosis factor alpha , hepatectomy , biology , receptor , medicine , cyclin d1 , regeneration (biology) , endocrinology , hepatocyte , mapk/erk pathway , microbiology and biotechnology , cell cycle , andrology , immunology , kinase , apoptosis , biochemistry , surgery , resection , in vitro
Aim: To investigate whether TNF‐ is necessary for hepatocyte proliferation, we study liver regeneration after partial hepatectomy in mice lacking TNF receptor‐1. Methods: TNF receptor type‐1 knockout mice and wild‐type mice were subjected to two‐thirds partial hepatectomy (PHx). Liver regeneration was evaluated by assessing liver weights and Ki67 immunohistochemistry. Riken cDNA microarray analysis was performed on liver samples from mice undergoing PHx to compare clearly differentiated mouse PHx models (TNFR‐1 knockout mice‐K group, and wild type mice‐W group). Results: The cumulative survival after PHx in K group was lower than in W group. The mortality rate in K group during the first 3 days after PHx was higher (33%) than in W group. The time to regain the liver weight in K group was 14 days and 7 days in W group. The plasma IL‐6 levels in K type at 3 hr was significantly higher than in W group. The Ki67 expression in K group at 4 days was lower than in W group. LPS, Toll like receptor 4 precursor and MAPK 8 interacting protein in K group was higher than in W group. For cell cycle‐regulated genes, cyclin D1, NFB light chain and TNF receptor super family membrane 1a in K group was lower than in W group. Conclusions: Lack of TNF‐ signaling through TNF receptor type 1 suppresses liver regeneration after partial hepatectomy in spite of enhancement of LPS–JNK pathway, no TNF‐a and IL‐6 pathway.