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Comparison Between Omental Implantation and Omental Patch for Perforated Acetic Acid‐Induced Gastric Ulcer in Rats
Author(s) -
Matoba Yasunori,
Tokunaga Akira,
Moriyama Yukichi,
Iida Shinya,
Matsukura Norio,
Tajiri Takashi,
Katayama Hironori
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130116aq.x
Subject(s) - basic fibroblast growth factor , granulation tissue , medicine , wound healing , immunohistochemistry , pathology , angiogenesis , fibroblast , infiltration (hvac) , growth factor , receptor , surgery , biology , cell culture , genetics , physics , thermodynamics
Aim: Omental implantation, a surgical procedure in which a perforated gastric or duodenal ulcer is repaired by drawing and implanting a portion of the omentum into the digestive tract, accelerates ulcer healing and inhibits ulcer recurrence compared with omental patch from clinical results. To clarify these mechanism and differences, we investigated ulcer healing in two groups. Methods: In two groups of rats in which acetic acid‐induced gastric ulcers were perforated. Omental implantation was used for repaired in one group and omental patch was employed in the other group. Basic fibroblast growth factor (bFGF) mRNA‐positive cells were identified and localized by in situ hybridization. Fibroblast growth factor receptor (FGFR)‐positive cells were identified and localized by immunohistochemical analysis. Results: Antiinflammatory and angiogenic activity and accelerated collagen synthesis were seen in the omental implantation group. BFGF mRNA‐positive cells (macrophages, fibroblasts, and endothelial cells) and FGFR‐positive cells were seen within the omentum, resulting in abundant collagen production and rapid epithelial regeneration. In the omental patch group, extensive neutrophilic infiltration and ulcer recurrence were seen. Few bFGF mRNA‐positive cells and FGFR‐positive cells were seen within the omentum, resulting to inhibit omentum becoming to be granulation tissue and ulcer healing. Conclusions: These results indicated that omental implantation accelerated ulcer healing, and the presence of bFGF mRNA and FGFR played a significant role in this phenomenon.