Premium
Viability and Function of Autologous and Allogeneic Fibroblasts Seeded in Dermal Substitutes After Implantation
Author(s) -
Morimoto Naoki,
Suzuki Shigehiko,
Saso Yasumi,
Tomihata Kenji,
Taira Tsuguyoshi,
Takahashi Yoshitake,
Morikawa Noriyuki
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130116ap.x
Subject(s) - wound healing , contraction (grammar) , fibroblast , in vivo , in vitro , transplantation , medicine , chemistry , surgery , pathology , andrology , biology , biochemistry , microbiology and biotechnology
Aim: The Aim of this study is to investigate the viability and distribution in vivo of autologous and allogeneic fibroblasts seeded in the collagen sponges after implantation and to clalify which is more effective for wound healing. Methods: Three full‐thickness wounds were created on the backs of guinea pigs and acellular substitute, substitute seeded with autologous fibroblasts at the density of 1,0 × 10 6 cells/cm 2 , and substitute seeded with allogeneic fibroblasts at the same density were transplanted. Before implantation, fibroblasts were labeled with PKH26.1, 2, 3 weeks after implantation, the epithelized areas were measured and specimens were taken. Results: PKH26 labeled autologous and allogeneic fibroblasts remained viabl. In the wound covered with autologous fibroblasts seeded substitute, the epithelization was fastest, and wound contraction was minimal. In contrast, in the wound covered with allogeneic fibroblasts, the epithelization was latest and the contraction was largest Conclusions: Allogeneic fibroblasts seeded in the collagen sponge took and remained viable on the grafted area, but adversely affected the wound healing.