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symposium i‐2. pathophysiology and treatment of pressure ulcer
Moderated by Toshimasa Asahara: Sterilized Acellular Allogeneic Dermal Matrix for Scaffold of Cultured Composite Skin
Author(s) -
Guo Jingping,
Takami Yoshihiro,
Yamaguchi Ryo,
Shiota Noriko,
Ogo Ken,
Harii Kiyonori
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130116ae.x
Subject(s) - sterilization (economics) , scaffold , dermis , regeneration (biology) , transplantation , chemistry , medicine , biomedical engineering , surgery , pathology , microbiology and biotechnology , biology , foreign exchange , monetary economics , economics , foreign exchange market
Aim: Acellular Allogeneic Dermal Matrix ADM has been used for scaffold of cultured composite skin. However, possibility of disease transmission by ADM remains as its potential risk. So it is important to reduce the risk by ADM transplantation the purpose of this study was to evaluate whether ADM could be used as a scaffold of cultured composite skin safely. Method: Four different kinds of methods were used for sterilization, such as 98% glycerol, Pasteurization, Gamma irradiation, and ethylene oxide gas sterilization (EOG). Histological, immunohistochemical and electrophoretic (SDS‐PAGE) properties were evaluated. The property as a scaffold of cultured composites skin was evaluated using normal human cultured keratinosytes. Results: Four methods did not induce apparent collagen degradation of ADM on SDS‐PAGE. However, damage of basement membrane structure was observed by ADM treated with Pasteurization, Gamma irradiation, and EOG histological. These three methods also resulted in the poor regeneration of the epidermal layer in cultured composite skin. Conclusion: The results suggested that glycerol treatment was found to be the best method of ADM sterilization as far as its scaffold was concerned.