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Recombinant G‐CSF Induces Downregulation of IL‐12 Transcription After Partial Hepatectomy
Author(s) -
Okiyama J,
Oishi K,
Hayamizu K,
Aihaiti X,
Ohmori I,
Yoshimitsu M,
Itamoto T,
Asahara T
Publication year - 2005
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2005.130116ab.x
Subject(s) - recombinant dna , downregulation and upregulation , messenger rna , liver regeneration , transcription (linguistics) , hepatectomy , andrology , microbiology and biotechnology , immunity , chemistry , biology , regeneration (biology) , endocrinology , immunology , medicine , immune system , gene , biochemistry , surgery , resection , linguistics , philosophy
Aims: Recent studies have demonstrated that administration of recombinant IL‐12 induces cytotoxicity against regenerating hepatocytes. We measured transcription levels of IL‐12 in the remnant liver in a model of partial hepatectomy (PHx) to elucidate the mechanism of already reported facilitation of liver regeneration by recombinant human G‐CSF (rhG‐CSF). Methods: After PHx of F344 rats, total RNA of the remnant liver was extracted and mRNA levels of IL‐12p35 and G‐CSF were quantified by RT‐real time PCR. Results: G‐CSF mRNA levels increased and IL12p35 mRNA levels decreased at 6 and 20 hours after PHx (P < 0.01). Pre‐PHx rhG‐CSF treatment significantly further down‐regulated IL12p35 mRNA levels at 20 hours (P < 0.01). Conclusions: This result suggests that rhG‐CSF pretreatment accelerates tissue repair of the liver at least partially through regulation of cellular immunity.