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P‐II‐03
Effect of Platelet Derived Wound Healing Factor (PDWHF) on the Angiogenesis in the Cases of Artificial Dermis Grafts
Author(s) -
Kazutaka Soejima,
Chen Xin,
Masayuki Yamato,
Teruo Okano,
Motohiro Nozaki
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.abstractt-t.x
Subject(s) - dermis , angiogenesis , wound healing , medicine , platelet , artificial skin , neovascularization , surgery , pathology , anatomy , immunology , cancer research
Aim: The effect of platelet derived wound healing factor (PDWHF) with or without cultured cells on angiogenesis in the cases of artificial dermis grafts was investigated. Methods: Wistar strain rats were used for this study. The PDWHF was prepared from platelets. The endothelial cells and fibroblasts were prepared from thoracic aorta and back skin respectively. Then 2 experimental models were designed. 1) Full‐thickness wounds were created on back of the animals. Artificial dermis (TERUDERMIS ® , TERUMO Co, Japan) were grafted to the wounds. 2) Femoral artery and vein were isolated as a vascular pedicle and wrapped with a folded sheet of the AD (1 × 2 cm). Prior to AD grafts, the AD were treated with the PDWHF and/or cultured cells. Results: The combination use of the PDWHF and cultured cells showed best angiogenesis in both experimental models. Conclusions: The result of the present study revealed that treatment with PDWHF combined with cultured cells accelerate the wound angiogenesis.