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S‐IV‐03
Ectopic Bone Formation Accelerated by Human Mesenchymal Stem Cells and Osteogenic Cytokines via Nutrient Vessel Injection in Nude Rat
Author(s) -
Fukui M,
Akita S,
Akino K
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.abstractk.x
Subject(s) - mesenchymal stem cell , vascularity , wound healing , medicine , fibrosis , stem cell , transplantation , angiogenesis , basic fibroblast growth factor , bone healing , pathology , surgery , growth factor , microbiology and biotechnology , cancer research , biology , receptor
Critically larger bone and skin defects often lack the enough nutrient blood supply to induce normal wound healing. Additionally, deteriorated environment such as poor vascularity due to the hard fibrosis after irradiation or extensive tissue loss due to the highly damaged tissue or severe bacterial contamination, leads to finding another donor sites or another methods to repair. Potential free vascularized flaps are widely accepted for reconstruction purpose, however, the donor‐site morbidity is sometimes concerned and its clinical application may be limited. Tissue engineered tissues are now most actively investigated and partially clinically appropriate for use. In order to assist the bone wound healing, the human mesenchymal stem cells (hMSCs), wrapped with the abdominal fascial flap, which is nutrient of superficial epigastric vascular systems in bone, and the full‐thickness dorsal defect covered by artificial skin substitute as a carrier in skin, are used as cell source with potential differentiation with specific cytokines in a nude rat model for eliminating T cell immunity. The hMSCs and osteogenic cytokines such as bone morphogenetic protein‐2 (BMP‐2) and basic fibroblast growth factor (bFGF) were intra‐arteriallly injected and incubated for 10 minutes and then the gelatin‐carrier were wrapped with the abdominal superficial fascia and investigated for the subsequent experiments. The heterotopic bone formation was most significantly observed in 4 weeks after injection and this may be used clinically to enhance the compromised bone healing.