Premium
S‐II‐02
The Effects of IL‐10 and TNFα on Wound Healing of Intestinal Anastomosis
Author(s) -
Akito Moroguchi,
Ken Ishimura,
Keiichi Okano,
Taijiro Miyake,
Takashi Maeba,
Hajime Maeta
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.abstracte.x
Subject(s) - wound healing , extracellular matrix , tumor necrosis factor alpha , anastomosis , immunohistochemistry , matrix metalloproteinase , medicine , immunofluorescence , fibroblast , andrology , pathology , immunology , chemistry , surgery , in vitro , antibody , biochemistry
Aim: This study aimed to define the participation of local expression of IL‐10 and TNFα around the anastomotic segment and clarify the effects of IL‐10 and TNFα on anastomotic wound healing after digestive surgery under septic condition. Methods: Experimental animals were divided into LPS and control groups, which had either LPS or normal saline solution injected into the peritoneal cavity 24 h before transection and anastomosis of the colon. Immunohistochemical staining for IL‐10 and TNFα on tissue samples were examined after the operation. Fibroblasts were cultured with IL‐10 and TNFα, then proliferation rates were determined using the MTT assay. Type I collagen protein and MMP‐I were detected by indirect immunofluorescence. Results: In the LPS group, IL‐10 and TNFα expression were more enhanced than that in the control group 24 h after the operation. IL‐10 reduced fibroblasts proliferation in dose dependent fasion in the presence of TNFα. Indirect immunofluorescence showed that IL‐10 reduced type I collagen protein and increased MMP‐I. Conclusions: Local expression of IL‐10 and TNFα at the anastomotic site acted as an inhibitory factor in the wound healing process. IL‐10 suppressed the remodeling of the extracellular matrix during wound healing.