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Effects of Granulocyte Colony‐Stimulating Factor in Rats with Severe Acute Pancreatitis
Author(s) -
HongFang Tuo,
Masanobu Nakashima,
Nobutsugu Abe,
Masanori Sugiyama,
Yutaka Atomi
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.abstractac.x
Subject(s) - acute pancreatitis , phagocytosis , medicine , ascites , pancreatitis , granulocyte colony stimulating factor , granulocyte , colony stimulating factor , endocrinology , immunology , biology , stem cell , chemotherapy , haematopoiesis , genetics
Aim: It is reported that Granulocyte colony‐stimulating factor (G‐CSF) increases the number and functions of the neutrophils of blood in animal models. In the present study, we observed the effects of G‐CSF in rats with severe acute pancreatitis. Methods: Pancreatitis was induced by injection of 0.2 ml of 3% taurocholate acid into biliopancreatic duct of the Wistar rat. Thirty rats were randomized into three groups. 1: Control group (C group). 2: Acute pancreatitis (AP) group. 3: AP + G‐CSF groups. G‐CSF was administrated via jugular veins 1 hour before induction of pancreatitis. Blood and ascites at 1 and 3 hours after induction of pancreatitis were measured for the number of neutrophils, their phagocytosis and bactericidal activity and the concentrations of TNF‐á, IL‐6 and IL‐1â. Results: The phagocytic neutrophils increased in AP + G‐CSF group (22 ± 4.3 × 10 5 ) compared with AP group (10 ± 1.9 × 10 5 )(p < 0.05); the bactericidal neutrophils increased in AP + G‐CSF group (60 ± 14.5 × 10 5 ) compared with AP group (20 ± 6.1 × 10 5 )(p < 0.05). G‐CSF did not increase any the concentration of TNF‐á, IL‐6 and IL‐1â in blood and ascites. Conclusions: G‐CSF increases the number of phagocytic and bactericidal neutrophils of blood and ascites in SAP rat, without increasing the concentration of TNF‐á, IL‐6 and IL‐1â, therefore improves host defense against infection.