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Inflammation‐induced Angiogenesis: Potential Roles of IL‐8. and VEGF
Author(s) -
Yao M.,
Dueck M.,
MartinsGreen M.
Publication year - 2004
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/j.1067-1927.2004.0abstractxl.x
Subject(s) - angiogenesis , inflammation , wound healing , infiltration (hvac) , neovascularization , vascular endothelial growth factor , monocyte , antibody , immunology , medicine , blood vessel , vegf receptors , physics , thermodynamics
Inflammation is invariably accompanied by angiogenesis; both are important in wound healing. However, it is not known how angiogenic factors that are present during the inflammatory phase of wound healing are involved in inflammation‐induced angiogenesis. In this study, we address the contribution of two such factors, interleukin‐8 (IL‐8/CXCL8) and VEGF, in inflammation‐induced angiogenesis. We determined the relative levels of these molecules during the first two weeks of the wound healing process in partial‐thickness skin wounds of rabbits. IL‐8 levels increase rapidly after wounding, reaching maximal levels after 24 hr; in contrast, VEGF levels peak at 3 days. We also determined the time course of the infiltration of various inflammatory cells and production of new blood vessels after wounding. Neutrophils were markedly increased 4 hr after wounding, and their level peaked at 24 hr. Monocyte infiltration peaked 48 hr after wounding and decreased by day 3. The number of blood vessels was significantly increased by day 3 after wounding and continued to increase through days 5 and 7. In order to determine the contribution of IL‐8 and VEGF to the angiogenic process, we performed the wounding experiments in the presence and absence of IL‐8 and VEGF antibodies. Treatment with IL‐8 antibodies blocked the early stage of blood vessel formation but did not alter the late stage; in contrast, VEGF antibodies blocked the late stage without altering the early stage. Currently, we are performing studies using inhibitors against IL‐8 and VEGF receptors to determine whether we observe similar results; we are also beginning to decipher molecular mechanisms involved in this process. This study suggests that IL‐8 and VEGF play complementary roles in stimulating angiogenesis during wound healing.